Biology IX Practical Notebook – Allied School Official Edition

The Biology Class 9 Practical Notebook is an essential laboratory companion for all matriculation students studying biology. Published by Learning Support International (LSI), this comprehensive practical workbook contains all mandatory experiments as per Punjab Textbook Board (PCTB) curriculum requirements.

Complete Table of Contents:

Experiment 1: Introduction to Biology

Experiment 1.1: Study of different types of bacteria with the help of prepared slides and of Amoeba, Paramecium, Volvox from prepared slides/fresh culture/charts (Page 11)
Experiment 1.2: Study of external morphology of mustard plant and microscopic examination of root, stem, leaf, flower, fruit and seeds (Page 28)
Experiment 1.3: Identification of major organs and organ systems in a dissected frog (Dissection by demonstrator/teacher) (Page 54)

Experiment 3: Biodiversity

Experiment 3.1: Recognition of different plants and animals from fresh and preserved specimens on the basis of apparent distinguishing taxonomic characters (Page 74)
Experiment 3.2: Evaluation of graphs of a population of an insect (endangered due to excessive use of insecticides) and interpret the reasons for its endangered status (Page 99)

Experiment 4: Cells and Tissues

Experiment 4.1: Use of microscope to observe movement of small objects (Page 103)
Experiment 4.2: Microscopic examination of an animal cell (e.g. from frog’s blood) and a plant cell (e.g. from onion epidermis) using an appropriate temporary technique, such as iodine or methylene blue (Page 107)
Experiment 4.3: Identification of different parts of the cell from fresh preparations (Page 118)
Experiment 4.4: Preparation of the wet mounts of tissue from flowering plants and study of plant and animal tissues from charts and prepared slides (Page 123)
Experiment 4.5: Determination of the effect of tonicity on plasmolysis and deplasmolysis in plant cells or in red blood cell (Page 147)
Experiment 4.6: Data collection on the number of stomata per unit area on various plant leaves that grow in areas of differing humidity and compilation of data and graph it to determine the relationship between the variables (Page 153)
Experiment 4.7: To prove the process of osmosis by an experiment and explain it with the help of a labeled diagram (Page 159)

Experiment 5: Cell Cycle

Experiment 5.1: Observation of various stages of mitosis and meiosis by slides, model and charts (Page 166)
Experiment 5.2: Preparation of root tip squashes and study of the stages of mitosis (Page 178)

Experiment 6: Enzymes

Experiment 6.1: To demonstrate working of an enzyme in vitro e.g., pepsin working on meat in test tube (Page 185)
Experiment 6.2: Experiment to test enzyme action by putting diastase in a starch solution in test tube at 37°C and after fifteen minutes performing iodine test for presence of starch (Page 191)

Experiment 7: Bioenergetics

Experiment 7.1: To demonstrate the process of photosynthesis using an aquatic plant, Hydrilla (Page 196)
Experiment 7.2: Identification and labeling of the cellular and tissue structure in the CS of a leaf through observation under the microscope (Page 202)
Experiment 7.3: To investigate the necessity of chlorophyll, light and carbon dioxide for photosynthesis (Page 209)
Experiment 7.4: To demonstrate the process of respiration in germinating seeds by using limewater (Page 224)
Experiment 7.5: To investigate the release of carbon dioxide and heat during aerobic respiration in germinating seeds (Page 229)

Experiment 8: Nutrition

Experiment 8.1: Food tests: Benedict’s test for reducing sugar, iodine test for starch, spot test and emulsion test for fat, and Biuret test for protein in solution (Page 234)
Experiment 8.2: Microscopic examination of a transverse section of the small intestine to show the villi (Page 240)

Experiment 9: Transport

Experiment 9.1: Measurement of differences in length/weight of raw potato strips in concentrated salt solution and water (Page 245)
Experiment 9.2: Observation of root hairs on a growing root of onion, carrot etc (Page 250)
Experiment 9.3: Microscopic observation of the structure and number of stomata in an epidermal peel of a leaf (Page 255)
Experiment 9.4: To investigate of the rate of water loss at the two surfaces of a leaf by using cobalt chloride paper (Page 260)
Experiment 9.5: To investigate transpiration in potted plant under a bell jar (Page 264)
Experiment 9.6: Identification of xylem and phloem tissues in the prepared slides of stem, root and leaf (Page 268)
Experiment 9.7: To investigate the pathway of water in parts of plant using a suitable stain and compare sizes of various types of cells (Page 273)
Experiment 9.8: Identification of red and white blood cells under the light microscope on prepared slides and photomicrographs (Page 278)
Experiment 9.9: To investigate the effect of physical activity on pulse rate (Page 284)
Experiment 9.10: Experiment to show the capillary flow in a fishtail or fin or frog’s web (Page 291)

Key Features: ✓ Published by Learning Support International (LSI) ✓ Official Allied School recommended practical notebook ✓ Covers all 9 chapters with 30+ experiments ✓ Detailed experimental procedures and observations ✓ Space for diagrams, recordings, and results ✓ Aligned with Punjab Textbook Board curriculum ✓ High-quality paper for writing and drawing ✓ Durable binding for year-long laboratory use ✓ Page numbers for easy navigation (196+ pages) ✓ Includes microscopy, dissection, and chemical tests

Perfect For:

Allied School Class 9 Biology students
Matriculation Part-I examination preparation
Laboratory practical work documentation
Biology project recordings
Federal and Punjab Board students

What Makes This Notebook Special: This practical notebook goes beyond basic experiments. It includes advanced topics like biodiversity evaluation, enzyme activity testing, photosynthesis demonstration, cellular observation, and transport system investigation. Each experiment is designed to build scientific thinking and practical skills essential for SSC examinations.

Delivery Across Pakistan: We deliver to all major cities including Lahore, Karachi, Islamabad, Rawalpindi, Peshawar, Quetta, Faisalabad, Multan, Sialkot, Gujranwala, and other areas. Cash on delivery available.

Order your Allied School Biology IX Practical Notebook today and excel in your laboratory examinations!